Friday, December 2, 2016

Excitement at fever-pitch in Oamaru #2

Where was the Only Man whom Goats Respect, when we needed him? It must have been his day off.

The level of Vitamin C within the stressed goat's liver was not recorded.

Below: Constable Conrad prepares blowpipes to fire tranquilliser darts for self-defense against rampaging goat / human genetic aberration.

Saturday, November 26, 2016

A drug by the name of "World Without End"
#2 -- Sympathy for Debility

Peter Duesberg had an intriguing though not widely-accepted theory [Below, right]. It involved everyone else being wrong, a recurring theme in Duesberg's oeuvre. More specifically: all the money and effort spent researching oncoregulator and oncopromoter genes are wasted, because there are no oncogenes... instead, cancer is a state of accelerating chromosomal disrepair, or 'aneuploidy', with the karotype going all higgledy-piggledy.

Don't worry, this will not be part of the final exam.

So Marco Ruggiero -- to whom we are grateful as a reliable source of material for Riddled -- liked this idea, and has made it his own. And in this world of post- modernism and deconstructionism where authorial intent is a quaint outmoded notion, he is free to shift the meaning slightly... to "There is no such thing as an oncogene, and it is Bcl-2".

Duesberg's actual words remain the same, in a novel context, much in the manner of genes within the broken and reassembled aneuploid chromosomes of a cancer cell-line.

Source is Ruggiero's Bcl-2 entry in the Springer Encyclopedia of Cancer [Manfred Schwab ed.], 3rd Edition, 2011... I don't always read Cancer reference tomes, but when I do, it's the Springer Encyclopedia. Yellow highlighting is not the authors', and probably not mine either, though who can be sure in this mixed-up deconstructionist post-authorship world?
Secret of Immortality: Blood
transfusions from Centaurs
Now Duesberg happened to remark in passing on the immortality of the resulting disinhibited cell lines, as one might say that HeLa cells and Devil Transmissible Facial Tumour qua distinct biological entities are immortal for as long as there remain Tasmanian Devils to infect. But "immortality" is a word that captures one's full attention, much in the manner of "World domination" or "Free Beer" or "10% discount on Mrs Miggin's Samphire-&-Scallion Pasties". Our man Ruggiero became over-excited by the concept, as if this were the continuity-of-personal-experience kind of immortality that one achieves through not dying.
This was our first warning that Ruggiero is beginning to adopt the viewpoints of pathogens and disease processes, which is an admirable trait for characters in J.G. Ballard novels and Burroughs fictions, but one prefers the loyalty of one's personal doctor to be less divided.

So come with me now on a stroll down the road, around the corner and over the railway overbridge, to a different intellectual magisterium... the world of HIV and AIDS. Form an orderly line, and please, no straggling.

Here we find Woodman and Williamson, describing the evolution of the HIV viral population within a human host as they adapt to escape the host's immune response, at the cost of not replicating as fast as they would like:
After transmission, HIV evolves at a rapid rate driven by the immune pressure until the virus reaches a delicate survival balance: on one hand avoiding elimination through the development of cytotoxic T-cell immune escape mutations, and on the other sacrificing replication fitness as these mutations may come with a severe fitness cost to the virus.
The phrase "delicate survival balance" caught our man's eye, and untrammeled by notions of 'author' or 'meaning' he was free to repurpose the paper for his own narrative -- much as one take scissors to the edges of a jigsaw piece so it can fit into a different jigsaw puzzle --
-- a narrative in which HIV is evolving at the species level for co-existence with humankind.

This account of HIV as an apologia for the virus, it is the second warning sign for Ruggiero. The fictive realm is drawing him down into its maelstrom and he is becoming a character from David Cronenberg's cinematic practice. This sort of thing happens more and more often (what with the morphogenic flux's increasing intensity), and it never ends well, with grotesque body-melt transformations and aesthetic folios full of skin diseases and such. Run, Marco! Escape while you still can!
"It's my conceit that perhaps some diseases perceived as diseases which destroy a well-functioning machine, in fact change the machine into a machine that does something else, and we have to figure out what it is that the machine now does. Instead of having a defective machine, we have a nicely functioning machine that just has a different purpose. Part of it is a self-deceptive way of coping with the possibilities of disease, but on the other hand I can imagine what it feels like to be a virus. The AIDS virus: look at it from his point of view. Very vital, very excited, really having a good time. [...] A virus is a living creature - actually, sometimes they go crystalline on you, which is what's interesting. See the movies from the point of view of the disease. You can see why they would resist all attempts to destroy them."
I am not making this up. Attend once again to the Springer Encyclopedia, and Ruggiero on Viral Protein R, introducing his theory that HIV is an unjustly-maligned cancer-curing human symbiont:
"Whaddafuck is that garish migraine horror? The goggles do nothing!" exclaims the reader, recoiling, eyeballs bleeding in the throes of a tequila-hangover flashback, unfamiliar with postmodern deconstructionist conventions of colour-coded highlighting. The point is to show the secret life of text, and how genetic sequences migrate independently, crossing the species volume barrier in episodes of horizontal transfer.
Sequences from Mishra et al., J Biol Chem 282 (7), 4288-4300 (2006) *:

and Muthumani et al., Curr HIV Res. 2009 Mar;7(2):144-52:
-----------------------------------------------------------------
That would be a good place to stop, except I have filled in a requisition form for a lot of yellow and blue highlighter, and I might as well use them as it is not worth the extra trouble and form-filling just to send them back into the store-room again. So here are Zhang et al. [below, left], Hum. Mol. Genet. (2001) 10 (21): 2329-2339, making the point that proteins sentences of the Bcl-2 gene family literature are evolutionarily conserved from the Sponges original papers to man Encyclopedia entries. And below that an excerpt from the Whackyweedia.


-----------------------------------------------------------------
Here are Zinkel, Gross and Yang -- Cell Death and Differentiation (2006) 13, 1351–1359:
For comparison with the Encyclopedia entry:
-----------------------------------------------------------------
Presumably space limitations in the Encyclopedia prevented Ruggiero from crediting all his sources in the detail he would have preferred. It is unfortunate that he wasted one of his nine citations on Yamamoto et al. (2008), which is to say a discredited fabrication [three of the four key GcMAF-cures-cancer papers from Yamamoto were retracted, on account of made-up ethics clearance and the probable non-existence of patients; concerns about the Translational Oncology paper were ignored, because Elsevier].
The paragraph advertising Ruggiero's commercial GcMAF product is also a lamentable use of space, shoehorned rather awkwardly into the text with an admission that it has no actual link to the putative topic of the entry. Perhaps a Conflict-of-Interest disclosure would be a worthwhile addition to the next revision.

Speaking of which, there was in fact a 2015 revision of this 3rd Edition. The Bcl-2 entry gained a second author, John W. Anderson.

Now Anderson is not a scientist or researcher. He is more associated with the Isagenyx ziggurat scheme multi-level-marketing operation, which deals in food supplements and weight-loss pills. Anderson's "Dream Master Laboratory"** was employing Ruggiero in 2015, while Anderson's address was used to register Ruggiero's magic-yoghurt distribution company "Bacterix LLC". Comparing the two versions, it is not obvious what revisions or additions Anderson contributed to this updated entry to earn his co-authorship and the associated academic respectability, other than providing Ruggiero with employment, an affiliation, and accommodation.
-----------------------------------------------------------------
* The absence of a citation for Mishra et al. is lamentable, as it deprives readers of the opportunity to check the paper in question, and to discover that the claims it makes are not substantiated -- it was retracted in 2011 on account of manipulated data.

** Evidently a plant-extract importation company rather than an actual laboratory.

*** No longer extant -- recently deregistered in a state of tax delinquency.
-----------------------------------------------------------------
UPDATE:
The analogy between genetic and textual material goes further than I realised; just as genes can duplicate along the genome, leaving each copy to evolve separately to fill a different role, so can paragraphs on pp. 357 and 358.


Bonus SYMBIOSIS.
Ruggiero's fondness for the deriding approach goes back a long way [h/t Dora].

Wednesday, November 23, 2016

Clothing-optional day at the Old Entomologist

In retrospect, possibly not as good an idea as it seemed at the time.
I blame the fish. They were drugged or something.

Friday, November 18, 2016

Bohemian Rhapsody, part III

In this installment our hero encounters the horrors of English cuisine, and seeks employment in the service sector. Soon to be a block-buster production from the Riddled Amateur Dramatic Society!

If Jan Váňa were updating his Nový Kapesní Slovník for contemporary conditions he would include a translation do češtiny of "Feckin Eastern Euros comin here and stealin ah jobs".

Spectres of the Spectrum

Huzzah! Here is some sciency-sounding radiation-related batshit-craziness magical thinking that is not from Professor Michael Persinger! We should hate to become dependent upon a single supplier.

Dr Blank's reasoning is irrefutable in its geometric logic. Expose DNA to non-ionising radiation in the radio frequencies, which should only cause thermal warming, yet Maxwell's Silver Equation comes down upon its head, leaving the DNA with single-strand breaks. But DNA incurs the same damage from ELF waves... with wavelengths measured in continental distances... which is to say the control condition.

You might conclude that the effect is therefore spurious, but that is why you are not popular and very truly run after and cited as World's Leading Authority by every loon with an electrosmog allergy and a personal Faraday cage. Blank's genius was to deduce that DNA must be a Fractal Antenna, absorbing energy with equal efficiency at all wavelengths. As further proof of the unique antennal properties of DNA he adduces the purported ELF sensitivity of the Na,K-ATPase and Cytochrome C Oxidase reactions (which involve no DNA) and of the Belousov-Zhabotinsky spatial-temporal reaction (which isn't even biological).

The crucial implication here is that if Raquel Welch is shrunk down to nano-naut size and inserted into your bloodstream to perform life-saving internal surgery, she will simply need to connect her radio to the nearest chromosome with a couple of jumper leads when she needs to receive messages from Mission Control.
At Riddled Research Laboratories we were sufficiently inspired to test the effect on DNA of sonic energy. Our experiments showed that it is especially sensitive to sarcasm, dramatic irony, bathos and litotes. Side-effects of the genome modification included extreme facial ductility [below, left] and Zodiacal growths [right].

In other news, DNA is not a conductor.

I am also advised that the special geometry of fractal antennae does not improve their extreme inefficiency at wavelengths longer than the antennae dimension L (larger, in this case, by a factor of 10¹²). It does improve performance at shorter wavelengths, rather than being stuck with peak resonance for wavelengths 2L; so a properly-designed, conducting, chromosome-sized hyper-coiled coil would not be confined to detecting infrared photons with a 5-micron wavelength, but could also absorb more energetic light into the visible and ultraviolet ranges.

It remains for Aterini and Ruggiero to ridicule the whole concept by suggesting that the fractal antennae also work as transmitters and enable our cells to communicate with us on the radio bands. Though I am puzzled by their choice of the august pages of the Springer Encyclopedia of Cancer as their vehicle for this parodic humour. I hope it's parody.
Must go, Raquel Welch is sending messages from the vicinity of my Islets of Langerhans [Latitude 38° 54' N, Longitude 77° 00' 13" W].